The monoclonal antibody Ba103 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38kDa or ~74 kDa (reduced or non-reduced condition, respectively). CD200R3 presumably exists as adisulfide-linked dimer on the cell surface, it is a single-pass type I membrane protein.The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R containstwo Ig-like extracellular domains, one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type(immunoglobulin-like) domain. and mediates inhibitory signals in myeloid cells. Mice deficient for theligand CD200 develop enhanced experimental allergic encephalomyelitis and collagen-induced arthritis.Unlike CD200R, the CD200R-like receptors function as activating receptors. They contain shortcytoplasmic tails and a lysine residue in the transmembrane region and are likely to signal via adaptorproteins such as DAP12, DAP10, FcRg, or CD3z.CD200R3 is expressed in uterus and bone marrow-derived mast cells (at protein level). It is expressed inuterus, spleen, bone marrow-derived dendritic, basophil and mast cells. CD200R3 is also expressed inthe lung of N. brasiliensis-infected mice. It is weakly expressed in brain, testis, lung and thymus.CD200R3 is expressed preferentially on cells of the myeloid lineage, including mast cells and basophils.Both cell types are involved in the host defense system against pathogens and in the development ofallergic disorders. They function as independent essential initiators of allergic reactions. Basophils are theleast common leukocytes in the peripheral blood accounting approximately 0.5 % of all leukocytes. In vivodepletion of basophils by using monoclonal antibody Ba103 proved the essential role of basophils in thedevelopment and maintenance of IgE-mediated chronic allergic inflammation.The activating CD200 receptors, like CD200R3, play an important role in IgE-independent mast cell andbasophil activation. Monoclonal antibody Ba103 activates basophils ex vivo and elicits systemicanaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibodyBa103 induces degranulation. Furthermore, monoclonal antibody Ba103 is useful for depletion ofbasophils.
Hycult Biotech 是一家专注于设计、研发、生产以及销售抗体和其相关产品的公司,其特色产品是天然免疫及相关领域的免疫分析试剂,例如补体, 嗜中性粒细胞蛋白,TLP,清道夫受体以及急性期蛋白等。除此之外,Hycult Biotech公司也可以提供炎症反应和细胞损伤等方面的产品。
Hycult Biotech的宗旨和策略是着重于天然免疫相关疾病的研究,为人类生活质量的提高谋福利。作为免疫学和细胞生物学领域的领导者,Hycult Biotech公司不仅研发这些用于生物科学研究的产品,同时也致力于将这些科研产品转化为临床测试的研究。该公司与40多家主要的国际研究机构有着紧密的合作,因此,他们可以持续提供多种创新型试剂。Hycult Biotech公司在1988年就已经获得ISO-9001质量验证,2007年获得ISO-13485验证,2009年获得CE质量认证。Hycult Biotech公司凭借其先进的理念,高效的研究团队以及严格的质监系统为广大科研工作者提供高质量的产品及周到的技术服务。