Catalognumber | HM2174-IA |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 0.5mg |
Formulation | 0.5mgof0.2µmfilteredproteinGpurifiedantibodysolutioninPBS(exactconcentrationisindicatedonthelabel). |
Application | Immunoassays,Westernblot |
ApplicationNotes | IA:theantibodycanbeusedascaptureantibody.Antibody265-3K1wasuniqueinthatthelimitofdetectionasacaptureantibodyincreasedfrom625nM(18.8ng/ml)forfreeHNEto80nMforHNE–AATcomplex,indicatingthatcovalentcomplexformationbetweenHNEandAATincreasesantibodyaffinityorincreasesexposureofrecognisedepitopeinHNEfor265-3K1(Ref.1). FC:cellswerefixedwithADGfixationbufferAfor15minatroomtemperature.Cellswerewashedandincubatedwith10μgofmonoclonalanti-HNEantibodydilutedinADGpermeABIlisationbufferB(Ref.1). IF:tissueswerefixedinparaformaldehyde(PFA)3.7%,embeddedinparaffinandsectionedat6mm.Thesectionsweredeparaffinizedintolueneandhydratedingradedseriesofethanol(Ref.2). |
Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Isotype | MouseIgG1 |
Species | Human |
References | 1.Davies,Petal;Monoclonalanti-neutrophilelastaseantibodycharacterisation:abilitytoblockfunction,detectfreeversusserpin-complexedenzymeandstainintracellulargranules.JImmunol.Meth.2008,336:175 2.Delbosc,Setal;PorphyromonasgingivalisParticipatesinPathogenesisofHumanABDominalAorticAneurysmbyNeutrophilActivation.ProofofConceptinRats.PLosONE2011,6:e18679 3.Novak,Tetal;OralUlcerationinBehçet"sDisease:AnInvestigationofNeutrophilElastaseandItsInhibitors.Thesis2013 |
Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
Disease | Autoimmunity,Pulmonology |
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