Catalog number | HM1096BT |
---|---|
Product type | Monoclonal antibodies |
Quantity | 50 µg |
Formulation | 0.5 ml (100 μg/ml) 0.2 μm filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
Conjugate | Biotin |
Application | Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Western blot |
Application Notes | IHC-F: Antibody JL-1 was used to stain tissue sections which were fixed in acetone. As positive control a polyclonal anti-C1q antibody was used and as negative control an isotype matched monoclonal antibody (Ref.1). FS: Antibody JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used (Ref.1). |
Use | Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. |
Immunogen | Purified mouse C1q |
Isotype | Mouse IgG2b |
Species | Mouse |
Cross reactivity | Human - Yes, Rat - Yes |
Alias | Complement component C1q, Complement C1q subcomponent subunit A |
Positive Control | Spleen and kidney tissue of wild-type mice (Ref.1) |
Negative Control | Spleen and kidney tissue of C1q -/- mice (Ref.1) |
References | 1. Trouw, L et al; Anti-C1q autoantibodies deposit in glomeruli but are only pathogenic in combination with glomerular C1q-containing immune complexes. J Clin Invest 2004, 114: 679 2. Li, M et al; Development of a humanized C1q A chain knock-in mouse: assessment of antibody independent beta-amyloid induced complement activation. Mol Immunol 2008, 45: 3244 3. Erlich, P et al; Complement protein C1q forms a complex with cytotoxic prion protein oligomers. J Biol Chem 2010, 285: 19267 |
Storage and stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year. |
Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product. |
Disease | Nephrology, Neurological disorders |
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